همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

Gut Proteomics Reflecting Extraintestinal Organ Involvement in IBD

نویسندگان :

Leila Ghanbari Maman¹ Sadegh Rizi² Mahdi Anvari³ Anna Meyfour⁴ Kaveh Kavousi⁵

1- Laboratory of Complex Biological systems and Bioinformatics (CBB), Department of Bioinformatics, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran 2- Department of Biotechnology, University of Tehran, Tehran, Iran 3- Department of Biotechnology, University of Tehran, Tehran, Iran 4- Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran 5- Laboratory of Complex Biological systems and Bioinformatics (CBB), Department of Bioinformatics, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran

کلمات کلیدی :

Pediatric Inflammatory Bowel Disease (PIBD)،Extraintestinal Organ Involvment،Proteomics،Differentially Expressed Proteins،Enrichment Analysis

چکیده :

Inflammatory Bowel Disease, including Crohn's Disease (CD) and Ulcerative Colitis (UC), is a chronic gastrointestinal disorder characterized by inflammation. About 25% of IBD patients are pediatric (Rosen et al., 2015). Pediatric IBD is of concern due to aggressive progression, nutritional deficiencies, growth failure, and complications from early disease onset (Ishige, 2019). Additionally, pediatric IBD care is more costly than adult care (Kappelman et al., 2008). IBD affects not only the gastrointestinal tract but also other organs, referred to as extraintestinal complications. These can involve the musculoskeletal system, skin, hepatobiliary tract, and eyes, significantly impacting patients’ quality of life (Rogler et al., 2021). Also, extraintestinal complications present challenges for healthcare providers in managing IBD. We employed proteomics data from a cohort of pediatric patients, consisting of 22 CD cases, 18 UC cases, and 22 healthy controls from mucosal luminal interface samples of the colon (Zhang et al., 2018). Raw MS data were preprocessed using MaxQuant and differentially expressed proteins among CD, UC, and healthy controls were identified using the DEP package in R. We focused on proteins significantly altered in IBD patients compared to healthy controls as well as those distinguishing subtypes of IBD. Enrichment analysis was conducted to identify tissues and gene ontologies associated with the significant proteins. Our findings suggest that intestinal proteomics data reflects the potential links between other organs and IBD, which may offer insights into the broader physiological effects of the condition.