همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

Exploring Genetic Variability: A Bioinformatics Approach to Analyzing Reported SNPs in the cagA Gene of Helicobacter pylori

نویسندگان :

Aria Soltani¹

1- Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

کلمات کلیدی :

Helicobacter pylori،SNP،cagA

چکیده :

Introduction: Single nucleotide polymorphisms (SNPs) within the cagA gene of Helicobacter pylori influence the bacterium’s virulence by altering the amino acid sequence and phosphorylation sites of the CagA protein, affecting its interactions with host proteins. These variations are linked to clinical outcomes such as increased gastric cancer risk and severe inflammatory responses. Understanding cagA SNPs aids in unraveling H. pylori pathogenic mechanisms and developing targeted therapies and vaccines, emphasizing the need for personalized treatment approaches. Methods: In this study, SNPs in the cagA gene were identified by comparing the sequences obtained from 30 gastric biopsy samples of patients diagnosed with H. pylori infection to validated SNPs with known positions on the reference gene HP0547. The analysis of these sequences, which were acquired through next-generation sequencing, was conducted using BLAST to detect the SNPs. Results: The analysis revealed a mix of conserved and variable SNP patterns among the sequences. It was found that SNPs such as G2701A and G3100A were consistently present in all samples, while others, such as C1279A and C581T, were uniformly absent. Additionally, it was observed that some SNPs exhibited variable distribution; for instance, G154A was identified in only one sample, whereas A1280G was detected in all but one sequence. Discussion: Variations in SNPs within the cagA gene can result in structural and functional changes in the CagA protein, which is a critical virulence factor of H. pylori. An understanding of these genetic differences is provided, offering valuable insights for the tailoring of antibiotic treatments and the development of vaccines aimed at specific H. pylori strains prevalent in Iran. The need for region-specific studies to enhance