همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

Identification of circRNA-miRNA-mRNA Interaction in Myocardial Infarction

نویسندگان :

Amir Hesam Pahlevani¹ Ashkan Nazari² Kiarash Zare³ Mohammad Ghorbani⁴ Abdolhakim Aalkamel⁵ Mohammad Mehdi Naghizadeh⁶

1- دانشگاه علوم پزشکی فسا 2- دانشگاه علوم پزشکی فسا 3- دانشگاه علوم پزشکی فسا 4- دانشگاه علوم پزشکی فسا 5- دانشگاه علوم پزشکی فسا 6- دانشگاه علوم پزشکی فسا

کلمات کلیدی :

circRNA،miRNA،myocardial infarction،regulatory network

چکیده :

Myocardial infarction (MI) is a severe cardiovascular condition resulting from sudden blood flow blockage to a heart muscle, causing tissue damage and severe patient outcomes, prompting researchers to explore molecular mechanisms [1,2]. non-coding RNAs, such as miRNAs and circRNAs have recently gained attention due to their regulatory roles in gene expression [3]. Emerging evidence suggests that non-coding RNAs play crucial roles in cardiovascular diseases, including MI. Gene expression profiles (GSE24519 and GSE24548) were downloaded from the Gene Expression Omnibus. Differentially express genes and miRNA were identified using the GEO2R. Enrichr was used to obtain the pathway and biological process. miRTarbase and CircBank databases databases were used to receive miRNAs and circRNAs. The circRNA-miRNA-mRNA interaction was reconstructed with Cytoscape software. Functional enrichment analysis revealed that several pathways and biological process associated synthesis of PIPs at early endosome membrane, PI metabolism, adherens junctions interactions, protein deglutamylation, receptor catabolic process and cellular response to potassium ion. The results of this study showed that ZNF701, HOXA10, TSR1, miR-485-5p, miR-142-3p, miR-142-5p, circ_0087424, circ_0087425, circ_0087427 and circ_0087428 have most important regulatory role. This study identifies a circRNA-miRNA-mRNA interaction, offering insights into MI pathophysiology and potential biomarkers for managing this serious cardiac condition.