همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

Design and Analysis of siRNA for Silencing the NS3 Gene of Hepatitis C Virus: A Novel Therapeutic Approach

نویسندگان :

Manouchehr Teymouri¹ Sara Eslami²

1- دانشگاه بجنورد 2- دانشگاه بجنورد

کلمات کلیدی :

siRNA design،Therapeutic RNA silencing،Hepatitis C Virus (HCV)،NS3 helicase inhibition،RNA interference (RNAi)

چکیده :

This study focuses on the design and analysis of siRNA to inhibit the NS3 gene of the Hepatitis C Virus (HCV). The main objective was to identify mRNA sequences of the NS3 gene and design siRNAs to suppress its activity. Using NCBI, the NS3 gene sequence was identified, and the coding sequence of the helicase domain was selected due to its conserved nature for siRNA design. RNAstructure software was employed to simulate siRNA-mRNA interactions for the HCV genome. Additionally, BLAST and RNAhybrid tools were used to evaluate potential off-target effects on the human genome. These methods quantitatively predicted the targeting accuracy and efficacy of siRNAs in inhibiting HCV replication. The results demonstrate that the designed siRNAs exhibit significant potential as an effective therapeutic strategy for treating HCV infection.