همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

In silico analysis of Maize WRKY transcription factors in response to drought and salt stress

نویسندگان :

Majid NorouzI¹ Sahar Shahgoli² Bahram Baghban Kohnehrouz³

1- Department of Plant Breeding & Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. 2- Department of Plant Breeding & Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. 3- Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

کلمات کلیدی :

Drought stress،Salinity Stress،WRKY transcription factor

چکیده :

After wheat and rice, maize is the most important agricultural product and is very important for human, livestock, pharmaceutical and food industries. Biotic and abiotic stresses are the most important factors limiting the performance of agricultural plants. The response to stress in organisms usually occurs with changes in gene expression due to the intervention of transcription factors. The WRKY gene family encodes a large group of transcription factors that play a very important role in the regulation of genes responsive to biotic and abiotic stresses. In this research, a comprehensive analysis of maize WRKY transcription factors was investigated using a bioinformatics approach. For this purpose, the entire maize proteome was first obtained from the Ensemble Plants database and a total of 129 significant sequences corresponding to the non-repetitive HMM profile were obtained based on the HMM pattern of the WRKY domain. The highest gene density was found to be associated with chromosome numbers 8 and 3, respectively, with 25 and 23 WRKY genes. A total of 39,669 WRKY transcription factor binding sites were identified in the maize genome, of which 7,503 unique genes had at least one WRKY transcription factor binding site. Of the total 47 WRKY profiles introduced in the JASAPAR database for Arabidopsis, 37 profiles were identified in the maize genome that were significantly present in the maize genome. Based on the study of the phylogenetic relationships of the maize WRKY genes, the genes were classified into three groups. Group I genes, genes structurally, had two WRKY domains. Finally, based on the information available in gene expression databases, 52 WRKY genes were observed to be involved in drought stress and 26 genes in salt stress. Under drought stress, 31 genes were up-regulated and 21 genes were down-regulated in expression, and in the conditions of salt stress all genes have down-regulated.