0% Complete
صفحه اصلی
/
4th international edition and 13th Iranian Conference on Bioinformatics
Identification of Antigenic Proteins of Acinetobacter baumannii as Potential Novel Vaccine Candidates Through a Reverse Vaccinology Approach
نویسندگان :
Amirhossein Ghadiri
1
Abbas Doosti
2
Mostafa Shakhsi-Niaei
3
1- Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3- Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran
کلمات کلیدی :
Vaccine،Acinetobacter baumannii،Protein antigen،In silico
چکیده :
Infections caused by Acinetobacter baumannii (A. baumannii) are increasing in prevalence globally. Therefore, vaccination appears necessary as a cost-effective preventative measure against this bacterium. In the present study, we employed subtractive proteomics and reverse vaccinology approaches to identify potential therapeutics against A. baumannii. Utilizing the Vaxign online tool, we analyzed over 35 genomes of A. baumannii strains and selected outer membrane and secreted proteins as potential vaccine candidates. Investigations were conducted on the immunogenicity, antigenic characteristics, physicochemical properties, and epitope densities of B-cell and MHC class I and II molecules in the proteins. Following the optimization of the protein codons, the pcDNA3.1(+) expression construct was designed, and the immunogenicity, allergenicity, and physicochemical properties of the vaccine construct were predicted. The ompW protein is predicted to be extracellular and located in the outer membrane. Immunoassays of ompW indicated an antigenicity of 0.67. This protein has five structural cell epitope points and five linear B epitope points. Additionally, it can bind to various HLA alleles of MHC class I and class II, making it a selected immunogenic protein suitable for designing non-allergenic structures. The results of this in silico study demonstrate high specificity and the development of a robust humoral and cellular immune response. Therefore, it can be concluded that the ompW vaccine construct is a promising candidate against A. baumannii that requires further testing in vitro and in vivo to prove its efficacy.
لیست مقالات
لیست مقالات بایگانی شده
Molecular docking and bioinformatics study of active compounds of thyme) Thymus vulgaris( in inhibiting COX-2 enzyme related to inflammatory diseases
Razieh Biglari Farash - Azizollah Kheiry - Najmaddin Mortazavi - Mohsen Sani khani
Machine-learning based biomarker discovery for Striga resistance in sorghum
Leyla Nazari - Afshar Estakhr
Towards Efficient Recombinant Protein Production: In-silico screening of signal peptides for secretory production of recombinant factor C in mammalian cells
Maryam Latifi - Kosar Asadi - Mansoureh Shahbazi Dastjerdeh
Gut Proteomics Reflecting Extraintestinal Organ Involvement in IBD
Leila Ghanbari Maman - Sadegh Rizi - Mahdi Anvari - Anna Meyfour - Kaveh Kavousi
AcrB Inhibition: A Molecular Docking Approach to Combat Escherichia coli Infections
Faezeh Mohammadzadeh Habil - ٍElnaz Afshari
A New Method in Analyzing Drug-Drug Interaction Using Artificial Intelligence: Combination of Channel and Spatial Maps in Deep Attention Networks
Seyed Vahab Shojaedini - AmirReza Moeini
Investigation and comparison of TYDC2 gene expression in different tissues (root, leaf, capsule and petal) of medicinal plant poppy (Papaver somniferum L.) in terms of bioinformatics
Nazila Bagheri - Alireza Tarinejad - Mohammad Majidi - Karim Hasanpour
Improved COVID‐19 Diagnosis Using a Hybrid Transfer Learning Model with Fuzzy Edge Detection on CT Scan Images
Hassan Salarabadi - Mohammad Saber Iraji - Mehdi Salimi - Mehdi Zoberi
Identifying the expression profile of genes involved in human brain development using transcriptome data
Amirhosein Akhoondi - Mostafa Rafeipour - Farinaz Behfarjam
Bioinformatics analysis of the binding of various ligands to the acylhemoserine lactonase derived from Bacillus.
Nasim Forghani - Matia sadat Borhani - Zoheir Heshmatipour - Mahmoud Salehi - Mohadeseh Piri
بیشتر
ثمین همایش، سامانه مدیریت کنفرانس ها و جشنواره ها - نگارش 42.7.0