همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

Investigation and comparison of TYDC2 gene expression in different tissues (root, leaf, capsule and petal) of medicinal plant poppy (Papaver somniferum L.) in terms of bioinformatics

نویسندگان :

Nazila Bagheri¹ Alireza Tarinejad² Mohammad Majidi³ Karim Hasanpour⁴

1- دانشگاه شهید مدنی آذربایجان 2- دانشگاه شهید مدنی آذربایجان 3- دانشگاه شهید مدنی آذربایجان 4- دانشگاه تبریز

کلمات کلیدی :

Alkaloid،Gene expression،Sequencing،Poppy

چکیده :

Poppy with the scientific name Papaver somniferum L. belongs to the Papaveraceae family. More than 40 types of alkaloids are known in poppy, the most important of which are morphine, codeine, thebaine, noscapine and papaverine(Hayat et al., 2023). Alkaloids of the morphine group in poppy are synthesized from two amino acid L-tyrosine molecules through 17 enzymatic steps during several continuous oxidative reactions(Aghaali and Naghavi, 2024). The first key enzyme in this pathway is L-tyrosine decarboxylase (TYDC2). In this research, TYDC2 gene was compared and investigated in four tissues of poppy plant. The sequence of these tissues (root, leaf, capsule and petal) was identified by NGS method and studied bioinformatically, these sequences were selected from the Gene section of NCBI website and downloaded by SRAtoolkit tool and their quality was determined by the tool. fastqc was evaluated and gene expression graphs and heatmap graphs were drawn by Rstudio. Finally, it was analyzed and evaluated by gprofiler in terms of gene ontology. In this research, the expression level of the production gene was different in the mentioned tissues and the expression level was detected in the leaf tissue more than the other tested tissues, and the lowest expression level was observed in the root tissue. Finally, gene ontology, genes were also examined and it was found that; The molecular function and the extent of the biological process of this gene play a great role in the catalytic activity. It has been observed in various plant species that the biosynthesis of a large number of primary metabolites as well as secondary metabolites are dependent on this gene. Gene expression is a process in which the information contained in a gene is used to produce a functional product(Alharbi and Vakanski, 2023). Therefore, modeling and identifying the expression level of tissues helps significantly in the production of products with high efficiency, especially in the field of medicinal plants that have effective substances(Guo et al., 2024). This study can be useful for the production of important and essential alkaloids of poppy medicinal plant.