همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

Novel lncRNA‐miRNA‐mRNA competing endogenous RNA regulatory networks in glioma

نویسندگان :

Asoo Khani¹ Amir-Reza Javanmard²

1- دانشگاه تهران 2- دانشگاه تربیت مدرس

کلمات کلیدی :

ceRNA،Glioma،lncRNA،miRNA،mRNA interactions

چکیده :

Gliomas the most frequent type among primary brain tumors are highly heterogeneous. Understanding molecular mechanisms of glioma is crucial for developing effective therapies. Several altered signaling pathways and cross-linked relationships of ncRNAs and coding RNAs remain to be investigated. Identifying unknown interconnections between these genes may provide valuable clues for developing strategies for cancer therapy. The regulatory mechanisms of the ceRNA network in the pathogenesis of Gliomas remain to be investigated. Methods: In this study, we aimed to identify potential regulatory networks involved in glioma tumorigenesis, based on the ceRNA hypothesis. We obtained Four datasets (SRP434123, SRP233221, SRP328814, SRP114556) Which contain mRNA and lncRNA expression RNA-seq from 32 glioma tissues and 32 normal ones. Also, 2 and 12 samples contain miRNA-seq of glioma and 12 normal tissues, respectively. Following a series of analyses such as Survival and co-expression analysis, GO and KEGG enrichment analysis, using online tools including Targetscan, miRwalk, and mirDB the interactions between lncRNA, miRNA, and targeted mRNA were predicted and visualized using Cytoscape software. Conclusion: We constructed a regulatory network associated with glioma tumorigenesis we acquired five axes, "CRNDE/has-mir-223/STAB1", "CRNDE1/has-mir-150/TOP2", "NEAT1/has-mir-150/TOP2", "GRM3-AS1/has-mir-128/TOP2" and "GRM3-AS1/has-mir-128/STAB1" which were found to be related with the prognosis of glioma and present as potential ceRNA regulatory networks in glioma patients. Our results provided a potential regulatory network underlying glioma genesis and displayed that CRNDE can competitively bind to miR-223, and miR-150 also NEAT1 can competitively bind to miR-150 and miR-128. GRM3-AS1 acts as a ceRNA and binds to miR-128. Therefore, based on Functional Enrichment Analysis, modulating STAB1 and TOP2 expression levels in glioma, affects systemic lupus erythematosus (SLA), alcoholism, and Neutrophil extracellular trap pathways in glioma.