همایش ملی بیوانفورماتیک ایران

صفحه اصلی / 4th international edition and 13th Iranian Conference on Bioinformatics

Comprehensive integrated single-cell RNA sequencing analysis of brain metastasis and glioma microenvironment: Contrasting heterogeneity landscapes

نویسندگان :

Seyedeh Fatemeh Sajjadi¹ Najmeh Salehi² Mehdi Sadeghi³

1- 1. School of Biological Science, Institute for Research in Fundamental Sciences (IPM), Tehran, Iran 2- University of Tehran 3- 2National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

کلمات کلیدی :

brain metastasis،tumor microenvironment،single-cell RNA sequencing

چکیده :

Understanding the specific type of brain malignancy, source of brain metastasis, and underlying transformation mechanisms can help provide better treatment and less harm to patients. The tumor microenvironment plays a fundamental role in cancer progression and affects both primary and metastatic cancers. The use of single-cell RNA sequencing to gain insights into the heterogeneity profiles in the microenvironment of brain malignancies is useful for guiding treatment decisions. To comprehensively investigate the heterogeneity in gliomas and brain metastasis originating from different sources (lung and breast), we integrated data from three groups of single-cell RNA-sequencing datasets obtained from GEO. We gathered and processed single-cell RNA sequencing data from 90,168 cells obtained from 17 patients. We then employed the R package Seurat for dataset integration. Next, we clustered the data within the UMAP space and acquired differentially expressed genes for cell categorization. Our results underscore the significance of macrophages as abundant and pivotal constituents of gliomas. In contrast, lung-to-brain metastases exhibit elevated numbers of AT2, cytotoxic CD4+ T, and exhausted CD8+ T cells. Conversely, breast-to-brain metastases are characterized by an abundance of epithelial and myCAF cells. Our study not only illuminates the variation in the TME between brain metastasis with different origins but also opens the door to utilizing established markers for these cell types to differentiate primary brain metastatic cancers.

لیست مقالات

لیست مقالات بایگانی شده

Development of a Multi-Epitope Vaccine Candidate for Brucella: An Immunoinformatics Approach to Achieve Cross-Protection

Zohre Sadeghi - Zahra Davoudi

Comprehensive integrated single-cell RNA sequencing analysis of brain metastasis and glioma microenvironment: Contrasting heterogeneity landscapes

Seyedeh Fatemeh Sajjadi - Najmeh Salehi - Mehdi Sadeghi

Molecular Investigation of Periplasmic Sensor Histidine Kinase Interactions in Regulating UV Shield Formation of Cyanobacteria Nostoc Sp

Maryam Eskafi - Maryam Azimzadeh Irani

A Computational Approach to Identify Potent Inhibitors of Janus Kinase 1 from Natural Products: Structure-Based High-Throughput Virtual Screening and LightGBM Classifier.

Parisa Valipour

Predicting Anticancer Drug Repurposing Candidates using Knowledge Graphs

Marzieh Khodadadi AghGhaleh - Rooholah Abedian - Reza Zarghami - Sajjad Gharaghani

Novel Anti-ageing Strategy Via Targeting CST With Vitamin B1

SeyedMobin Mousavi Ghomi - Maryam Azimzadeh Irani - Aida Arezoumandchafi

Boosting Healing Through Stabilized Fibrin Clot: Structural Bioinformatics Exploration of Collagen’s Potential as a Sustained-Release Carrier for Tranexamic Acid

Mohammad Amin Rastegar - Sadegh Hasannia - Elnaz Tamjid

New inhibitors of the Toxoplasmosis by in-silico drug repurposing

Milad Jaberi - Masoud Aliyar - Mosleh Kadkhodamohammadi - Parva Karimimousivandi

ATIC as a Theranostic Biomarker in Gastrointestinal Cancers: Insights from Autophagy Pathway Analysis

Pooya Jalali - Maryam Eghbali - Zahra Eghabli - Yasaman Gholinezhad - Zahra Salehi

Identification of circRNA-miRNA-mRNA Interaction in Myocardial Infarction

Amir Hesam Pahlevani - Ashkan Nazari - Kiarash Zare - Mohammad Ghorbani - Abdolhakim Aalkamel - Mohammad Mehdi Naghizadeh

ثمین همایش، سامانه مدیریت کنفرانس ها و جشنواره ها - نگارش 40.4.1